The Seed Biology Place

Plant & Cell Physiology 53: 81-95 (2012)

**Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture**^[W]

Krystyna Oracz*, Antje Voegele, Danuše Tarkowská, Dominique Jacquemoud, Veronika Turecková, Terezie Urbanová, Miroslav Strnad, Elwira Sliwinska, Gerhard Leubner-Metzger*
*shared corresponding authors

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' - www.seedbiology.de (K.O., A.V., D.J., G.L.-M.)
Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, v.v.i., Šlechtitel? 11, CZ-783 71, Olomouc, Czech Republic (D.T., V.T., T.U., M.S.)
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Šlechtitel? 11, CZ-783 71, Olomouc, Czech Republic (M.S.)
Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics and Biotechnology, University of Technology and Life Sciences, Kaliskiego Ave. 7, PL-85-789 Bydgoszcz, Poland (E.S.)

Received 11 July 2011; Accepted 3 September 2011; First published online 8 September 2011
DOI 10.1093/pcp/pcr124

Supplementary Table S4 Main characteristics of genes and primer sequences for qRT-PCR used in the present work.

Abbreviation of gene	Description of gene	Specificity of primer	Accession number	Forward Primer: sequence 5' --> 3' Reverse Primer: sequence 5' --> 3'
SDR1/ABA2	cytosolic short-chain dehydrogenase/reductase	A. thaliana	At1g52340	F : ATGTGGAGCACCGTGCCCTG R : AACGCCTCCCACAACACCTCC
LesaCYP707A2	ABA 8'-hydroxylase CYP707A2	L. sativum	GQ221028 (At2g29090)	F : AAGAGCTTTCATGCCGGATTC R : AAGAGCTTTCATGCCGGATTC
LesaCYP707A3	ABA 8'-hydroxylase CYP707A3	L. sativum	GQ221029 (At5g45340)	F : ATCAACACCCTCGAACACATG R : TCAATTTCAGTGGCCTCCTCTT
LesaGID1a	GA receptor GIBBERLLIN INSENSITIVE DWARF 1a	L. sativum	HQ003455 (At3g05120)	F: AGCGGGTGAGTCTGGAATCAAT R: AAGTTTCTCAGACTCTGTTCTC
LesaGID1b	GA receptor GIBBERLLIN INSENSITIVE DWARF 1b	L. sativum	HQ003456 (At3g63010)	F: AGCTCACAATGTTGCTGTGAG R: TCAGTCCTCTCTTGTCCAC
LesaGID1c	GA receptor GIBBERLLIN INSENSITIVE DWARF 1c	L. sativum	HQ003457 (At5g27320)	F: GCATAATGTCGCGGTTAGAG R: GTCCCTCCAAACATAGGGTT
GA3ox1	GA3 oxidase 1	A. thaliana	At1g15550	F: TGGGCCCAAGCTGCTCTCCA R: AGCCAGGAAACGGTGGCACG
GA3ox2	GA3 oxidase 2	A. thaliana	At1g80340	F: GATGGACGTGGGCTGGGTTA R: CCCTGGCGGTGAAGCACG
GA3ox3	GA3 oxidase 3	A. thaliana	At4g21690	F: AACCCCGTGAACCGTGACCG R: ACGGGAATGGGTCCGCTCGT
GA3ox4	GA3 oxidase 4	A. thaliana	At1g80330	F: GTGGGCAGAGGCGCCATACG R: CCGGCTCAACCGTGACCCAA
GA2ox7	GA2 oxidase7	A. thaliana	At1g50960	F: GCAGCTGCGAAAGAGTGGGGA R: TGAGCGGGAGAAGTGGCGCT
Lesa17210	IPL1; IAP-like protein 1, zinc ion binding	L. sativum	HQ912755 (At1g17210)	F: TCCGCCCTTGTATGGACGAGA R: CTCTGGCACCTGGGAAAGCCA
Lesa20000	HBT; anaphase-promoting complex subunit	L. sativum	HQ912757 (At2g20000)	F: TCTGGTCCACGACGGAGCTTG R: TCTGGTCCACGACGGAGCTTG
Lesa04660	APC2; ubiquitin-protein ligase cell cycle regulation	L. sativum	HQ912754 (At2g04660)	F: AGCTGGGTCTATTGCACGAAG R: AGCTGGGTCTATTGCACGAAG

Article in PDF format (1.5 MB)
Supplementary data file (3.4 MB)

Abstract
Supplementary:

Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Table 1
Fig. S1 Fig. S2 Fig. S3 Fig. S4 Tab.S1 Tab.S2 Tab.S3 Tab.S4

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Plant & Cell Physiology 53: 81-95 (2012)

Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture[W]

**Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture**^[W]