The Seed Biology Place

Plant & Cell Physiology 53: 81-95 (2012)

**Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture**^[W]

Krystyna Oracz*, Antje Voegele, Danuše Tarkowská, Dominique Jacquemoud, Veronika Turecková, Terezie Urbanová, Miroslav Strnad, Elwira Sliwinska, Gerhard Leubner-Metzger*
*shared corresponding authors

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' - www.seedbiology.de (K.O., A.V., D.J., G.L.-M.)
Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, v.v.i., Šlechtitel? 11, CZ-783 71, Olomouc, Czech Republic (D.T., V.T., T.U., M.S.)
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Šlechtitel? 11, CZ-783 71, Olomouc, Czech Republic (M.S.)
Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics and Biotechnology, University of Technology and Life Sciences, Kaliskiego Ave. 7, PL-85-789 Bydgoszcz, Poland (E.S.)

Received 11 July 2011; Accepted 3 September 2011; First published online 8 September 2011
DOI 10.1093/pcp/pcr124

Supplementary Fig. S4. Selected DNA histograms (flow cytometry) of nuclear preparations from RAD zones isolated from dry Lepidium sativum seeds (A, D, G), control (B, E, H) and MyA (5x10-4 M My) treated (C, F, I) seeds imbibed for 22 h in continuous white light.

Results obtained from Zone 1 of RAD are presented on histograms A, B, C; from Zone 2 on histograms D, E, F; and from Zone 3 on histograms G, H, I. The localization of three distinct zones is shown on the Fig. 6 and the quantification is in Supplemental Table S2.

Article in PDF format (1.5 MB)
Supplementary data file (3.4 MB)

Abstract
Supplementary:

Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Table 1
Fig. S1 Fig. S2 Fig. S3 Fig. S4 Tab.S1 Tab.S2 Tab.S3 Tab.S4

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Plant & Cell Physiology 53: 81-95 (2012)

Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture[W]

**Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture**^[W]