The Seed Biology Place

Plant & Cell Physiology 53: 81-95 (2012)

**Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture**^[W]

Krystyna Oracz*, Antje Voegele, Danuše Tarkowská, Dominique Jacquemoud, Veronika Turecková, Terezie Urbanová, Miroslav Strnad, Elwira Sliwinska, Gerhard Leubner-Metzger*
*shared corresponding authors

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' - www.seedbiology.de (K.O., A.V., D.J., G.L.-M.)
Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, v.v.i., Šlechtitel? 11, CZ-783 71, Olomouc, Czech Republic (D.T., V.T., T.U., M.S.)
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Šlechtitel? 11, CZ-783 71, Olomouc, Czech Republic (M.S.)
Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics and Biotechnology, University of Technology and Life Sciences, Kaliskiego Ave. 7, PL-85-789 Bydgoszcz, Poland (E.S.)

Received 11 July 2011; Accepted 3 September 2011; First published online 8 September 2011
DOI 10.1093/pcp/pcr124

Fig. 4. The tissue-specific (RAD, CAP) effect of myrigalone A (MyA) on gibberellin (GA) metabolism and GA-related transcripts abundance during germination of Lepidium sativum seeds incubated in continuous white light.

(A) The 13-non-hydroxylated (in blue) and 13- hydroxylated (in red) GA biosynthesis and inactivation pathways and important metabolites detected in L. sativum seeds.

(B, C, D) Contents of bioactive GA such as GA1, GA4 and GA6, and inactive forms such as GA24, GA9, GA34, GA8, GA13 quantified in RAD and CAP excised from control (CON) and MyA-treated seeds incubated for 15 h.

(E) Normalized transcript abundance quantified by qRT-PCR in RAD and CAP of CON and MyA-treated seeds incubated for 15 h for GA3 oxidase (GA3ox1, GA3ox2, GA3ox3, GA3ox4) and GA2 oxidase (GA2ox7) genes, whose products catalyse activation and inactivation steps of bioactive GA as indicated in (A).

(F) Normalized transcript abundance for GA receptors of the GID1ac (LesaGID1a, LesaGID1c) and GID1b (LesaGID1b) groups. Mean values ± SE of 4 biological replicates.

Article in PDF format (1.5 MB)
Supplementary data file (3.4 MB)

Abstract
Supplementary:

Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Table 1
Fig. S1 Fig. S2 Fig. S3 Fig. S4 Tab.S1 Tab.S2 Tab.S3 Tab.S4

Webdesign Gerhard Leubner 2000
Best viewed with browser version 4 and 800x600 pixel
This page was last updated on 18 January, 2012

Plant & Cell Physiology 53: 81-95 (2012)

Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture[W]

**Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture**^[W]