Plant & Cell Physiology 53: 81-95 (2012)

Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture[W]
Krystyna Oracz*, Antje Voegele, Danuše Tarkowská, Dominique Jacquemoud, Veronika Turecková, Terezie Urbanová, Miroslav Strnad, Elwira Sliwinska, Gerhard Leubner-Metzger*
*shared corresponding authors
University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' - www.seedbiology.de (K.O., A.V., D.J., G.L.-M.)
Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, v.v.i., Šlechtitel? 11, CZ-783 71, Olomouc, Czech Republic (D.T., V.T., T.U., M.S.)
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Šlechtitel? 11, CZ-783 71, Olomouc, Czech Republic (M.S.)
Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics and Biotechnology, University of Technology and Life Sciences, Kaliskiego Ave. 7, PL-85-789 Bydgoszcz, Poland (E.S.)

Received 11 July 2011; Accepted 3 September 2011; First published online 8 September 2011
DOI 10.1093/pcp/pcr124
Supplementary Table S1.   Contents of active and inactive gibberellins (GA) in RAD and CAP
isolated from Lepidium sativum seeds imbibed for 15 h without (CON) or with 5x10-4 M MyA
added in continuous white light. The two major GA metabolic pathways, 13-non-hydroxylated
and 13-hydroxylated, are indicated.
Means ± SE of 4 biological replicates are presented and expressed in pg/mg dry weight.

   

RAD

  

CAP
   

CON

MyA

  

CON

MyA

13-Non-hydroxylation pathway

GA12ald

  

5.3 ± 2.2

0.0 ± 0.0

  

27.5 ± 16.1

0.0 ± 0.0

GA12

  

3.7 ± 0.2

5.7 ± 0.8

  

0.0 ± 0.0

3.8 ± 3.8

GA15

  

6.0 ± 2.4

6.7 ± 1.4

  

4.5 ± 4.5

16.9 ± 8.4

GA24

  

45.0 ± 1.8

36.2 ± 11.5

  

32.2 ± 9.5

45.1 ± 13.9

GA9

  

26.5 ± 3.5

5160 ± 652

  

13.9 ± 6.5

6450 ± 4016

GA51

  

4.2 ± 3.3

5.4 ± 3.7

  

44.5 ± 5.4

0.0 ± 0.0

GA4

  

56.8 ± 8.7

23.4 ± 2.9

  

26.4 ± 11.3

33.8 ± 22.9

GA34

  

2.3 ± 0.1

3.6 ± 0.8

  

4.6 ± 1.2

5.1 ± 0.9

GA13

  

6.2 ± 0.9

2.6 ± 0.9

  

53.4 ± 6.2

55.5 ± 13.8

13-Hydroxylation pathway

GA53

  

17.3 ± 4.3

4.8 ± 2.1

  

78.8 ± 10.2

30.0 ± 9.6

GA44

  

3.8 ± 2.2

3.6 ± 3.6

  

9.8 ± 6.1

8.5 ± 8.5

GA19

  

3.9 ± 2.4

2.6 ± 1.1

  

0.0 ± 0.0

0.0 ± 0.0

GA20

  

4.9 ± 2.2

1.6 ± 1.6

  

0.0 ± 0.0

0.0 ± 0.0

GA29

  

9.5 ± 3.2

10.8 ± 2.9

  

75.8 ± 13.7

17.0 ± 5.8

GA1

  

5.4 ± 1.2

4.4 ± 1.6

  

2.7 ± 2.7

0.0 ± 0.0

GA8

  

60.2 ± 2.7

50.2 ± 6.8

  

30.5 ± 20.9

18.6 ± 7.0

GA5

  

0.9 ± 0.9

0.0 ± 0.0

  

0.0 ± 0.0

0.0 ± 0.0

GA6

  

55.8 ± 4.8

36.6 ± 7.9

  

412.2 ± 41.3

20.8 ± 9.1

GA3

  

1.2 ± 0.1

0.9 ± 0.2

  

0.0 ± 0.0

0.4 ± 0.4

GA7

  

0.0 ± 0.0

0.0 ± 0.0

  

0.0 ± 0.0

0.0 ± 0.0

Article in PDF format (1.5 MB)
Supplementary data file (3.4 MB)
Abstract
Supplementary:
 Fig. 1         Fig. 2         Fig. 3         Fig. 4         Fig. 5         Fig. 6       Table 1
Fig. S1       Fig. S2       Fig. S3       Fig. S4       Tab.S1     Tab.S2     Tab.S3     Tab.S4
  
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