The Seed Biology Place

Plant & Cell Physiology 53: 81-95 (2012)

**Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture**^[W]

Krystyna Oracz*, Antje Voegele, Danuše Tarkowská, Dominique Jacquemoud, Veronika Turecková, Terezie Urbanová, Miroslav Strnad, Elwira Sliwinska, Gerhard Leubner-Metzger*
*shared corresponding authors

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' - www.seedbiology.de (K.O., A.V., D.J., G.L.-M.)
Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, v.v.i., Šlechtitel? 11, CZ-783 71, Olomouc, Czech Republic (D.T., V.T., T.U., M.S.)
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Šlechtitel? 11, CZ-783 71, Olomouc, Czech Republic (M.S.)
Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics and Biotechnology, University of Technology and Life Sciences, Kaliskiego Ave. 7, PL-85-789 Bydgoszcz, Poland (E.S.)

Received 11 July 2011; Accepted 3 September 2011; First published online 8 September 2011
DOI 10.1093/pcp/pcr124

Supplementary Table S3. Chemicals and concentrations used in germination assays with Lepidium sativum seeds.

Treatment	Final concentration	Origin (company, cat. no)
Myrigalone A (MyA)^a	0.1 µM to 1 mM	Prof. Gilles Comte, CESN, Lyon, France
Sulcotrione^b (synthetic herbicide)	10 nM to 1 mM	Fluka, cat. no 46318
Gibberellin A₄₊₇ (GA)	10µM	Duchefa, cat. no G0938
1-aminocyclopropane-1-carboxylic-acid (ACC)	1mM	Sigma, cat. no A3903
cis-S(+)-abscisic acid (ABA)	3 µM	Duchefa, cat. no A0941
α-pinene	1 mM	Aldrich, cat. no 14752-4
α-phellandrene	1 mM	Aldrich, cat. no W28560
p-cymene	1 mM	Aldrich, cat. no C12145-2
1,8-cineol	1 mM	Aldrich, cat. no C80601
1,4-cineol^b	1 mM	Fluka, cat. no 27393
Limonene^c	1 mM	Fluka, cat. no 62118

a) >99% purity; dissolved as a stock in methanol; therefore as control (CON) 0.35 (v/v) methanol was used in germination assays with L. sativum seeds.

b) Not present in M. gale fruits or leaves.

c) Identified in M. gale leaves, but not in fruit exudates.

Article in PDF format (1.5 MB)
Supplementary data file (3.4 MB)

Abstract
Supplementary:

Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Table 1
Fig. S1 Fig. S2 Fig. S3 Fig. S4 Tab.S1 Tab.S2 Tab.S3 Tab.S4

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Plant & Cell Physiology 53: 81-95 (2012)

Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture[W]

**Myrigalone A inhibits Lepidium sativum seed germination by interference with gibberellin metabolism and apoplastic superoxide production required for embryo extension growth and endosperm rupture**^[W]