The Seed Biology Place

Journal of Experimental Botany 63: 5337-5350 (2012)

**Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A** ^[W]^[OA]

Antje Voegele, Kai Graeber, Krystyna Oracz, Danuse Tarkowská, Dominique Jacquemoud, Veronika Turecková, Terezie Urbanová, Miroslav Strnad, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, German (AV, KG, KO, DJ, GLM)
School of Biological Sciences, Plant Molecular Science, Royal Holloway, University of London, Egham, Surrey TW20 0EX, Uk (AV, KG, GLM); Web: 'The Seed Biology Place' - www.seedbiology.eu
Department of Plant Physiology, Warsaw University of Life Sciences-SGGW, 15 Nowoursynowska 159, 02-776, Warsaw, Poland (KO)
Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, v.v.i., Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (DT, VT, TU, MS)
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (MS)

Received March 10 2012; Revised June 11 2012; Accepted June 15 2012
Advance Acess publication July 21 2012
DOI 10.1093/jxb/ers197

Table S1. Contents of active and inactive gibberellins (GA) and abscisic acid (ABA) in RAD and CAP isolated from Lepidium sativum seeds imbibed for 15 h without (CON) or with 5x10^-4 M MyA added either in continuous white light or in darkness. The two major GA metabolic pathways, 13-non-hydroxylated and 13-hydroxylated, are indicated. Means ± SE of 4 biological replicates are presented and are expressed in pg/mg dry weight for the GA metabolites and in pmol/g dry weight for the ABA metabolites.

Continuous white light

Darkness

RAD

CAP

RAD

CAP

CON

MyA

CON

MyA

CON

MyA

CON

MyA

13-Non-hydroxylation pathway

GA_12ald

5.3 ± 2.2

0.0 ± 0.0

27.5 ± 16.1

0.0 ± 0.0

13.8 ± 1.2

17.4 ± 13.7

187.4 ± 69.9

15.5 ± 15.5

GA₁₂

3.7 ± 0.2

5.7 ± 0.8

0.0 ± 0.0

3.8 ± 3.8

0.7 ± 0.7

0.0 ± 0.0

5.4 ± 5.4

4.3 ± 4.3

GA₁₅

6.0 ± 2.4

6.7 ± 1.4

4.5 ± 4.5

16.9 ± 8.4

5.9 ± 1.2

1.1 ± 1.1

10.2 ± 6.4

21.2 ± 7.6

GA₂₄

45.0 ± 1.8

36.2 ± 11.5

32.2 ± 9.5

45.1 ± 13.9

31.4 ± 5.9

9.3 ± 0.9

31.5 ± 10.8

52.6 ± 3.2

GA₉

26.5 ± 3.5

5160 ± 652

13.9 ± 6.5

6450 ± 4016

13.2 ± 4.6

5464 ± 631

11.4 ± 0.9

25820 ± 3062

GA₅₁

4.2 ± 3.3

5.4 ± 3.7

44.5 ± 5.4

0.0 ± 0.0

4.8 ± 4.8

4.9 ± 4.9

9.3 ± 5.4

27.2 ± 27.2

GA₄

56.8 ± 8.7

23.4 ± 2.9

26.4 ± 11.3

33.8 ± 22.9

45.0 ± 12.9

16.7 ± 7.5

42.9 ± 13.3

42.3 ± 6.0

GA₃₄

2.3 ± 0.1

3.6 ± 0.8

4.6 ± 1.2

5.1 ± 0.9

2.3 ± 0.9

1.7 ± 0.3

16.9 ± 13.1

7.2 ± 2.6

GA₁₃

6.2 ± 0.9

2.6 ± 0.9

53.4 ± 6.2

55.5 ± 13.8

2.4 ± 0.8

7.3 ± 2.9

89.9 ± 27.2

28.8 ± 14.3

13-Hydroxylation pathway

GA₅₃

17.3 ± 4.3

4.8 ± 2.1

78.8 ± 10.2

30.0 ± 9.6

10.1 ± 2.1

11.9 ± 2.8

46.6 ± 16.8

38.1 ± 6.9

GA₄₄

3.8 ± 2.2

3.6 ± 3.6

9.8 ± 6.1

8.5 ± 8.5

0.0 ± 0.0

GA₁₉

3.9 ± 2.4

2.6 ± 1.1

0.0 ± 0.0

2.3 ± 1.3

2.7 ± 1.6

0.0 ± 0.0

GA₂₀

4.9 ± 2.2

1.6 ± 1.6

0.0 ± 0.0

GA₂₉

9.5 ± 3.2

10.8 ± 2.9

75.8 ± 13.7

17.0 ± 5.8

4.3 ± 2.0

13.4 ± 6.4

47.4 ± 20.7

55.3 ± 25.5

GA₁

5.4 ± 1.2

4.4 ± 1.6

2.7 ± 2.7

0.0 ± 0.0

9.5 ± 3.8

2.8 ± 2.1

0.0 ± 0.0

GA₈

60.2 ± 2.7

50.2 ± 6.8

30.5 ± 20.9

18.6 ± 7.0

46.9 ± 7.8

26.0 ± 14.5

36.2 ± 5.6

41.7 ± 36.0

GA₅

0.9 ± 0.9

0.0 ± 0.0

GA₆

55.8 ± 4.8

36.6 ± 7.9

412.2 ± 41.3

20.8 ± 9.1

12.2 ± 6.2

40.6 ± 16.6

44.9 ± 11.6

254.7 ± 74.2

GA₃

1.2 ± 0.1

0.9 ± 0.2

0.0 ± 0.0

0.4 ± 0.4

0.1 ± 0.1

1.4 ± 1.3

1.8 ± 1.2

1.6 ± 0.6

GA₇

0.0 ± 0.0

0.1 ± 0.1

0.0 ± 0.0

Abscisic acid (ABA) and ABA-glucoseester (ABA-GE)

ABA

19.5 ± 1.6

28.7 ± 1.8

33.3 ± 4.7

55.6 ± 8.7

23.4 ± 0.6

31.5 ± 2.6

61.6 ± 3.8

45.9 ± 2.0

ABA-GE

201.2 ± 15.4

174.3 ± 27.5

129.5 ± 15.4

144.6 ± 35.0

215.7 ± 35.3

209.1 ± 37.7

172.4 ± 22.7

169.5 ± 29.1

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Abstract
Supplementary:

Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Fig. 7
Fig. S1 Fig. S2 Fig. S3 Fig. S4 Table S1

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Journal of Experimental Botany 63: 5337-5350 (2012)

Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A [W][OA]

**Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A** ^[W]^[OA]