The Seed Biology Place

Journal of Experimental Botany 63: 5337-5350 (2012)

**Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A** ^[W]^[OA]

Antje Voegele, Kai Graeber, Krystyna Oracz, Danuse Tarkowská, Dominique Jacquemoud, Veronika Turecková, Terezie Urbanová, Miroslav Strnad, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, German (AV, KG, KO, DJ, GLM)
School of Biological Sciences, Plant Molecular Science, Royal Holloway, University of London, Egham, Surrey TW20 0EX, Uk (AV, KG, GLM); Web: 'The Seed Biology Place' - www.seedbiology.eu
Department of Plant Physiology, Warsaw University of Life Sciences-SGGW, 15 Nowoursynowska 159, 02-776, Warsaw, Poland (KO)
Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, v.v.i., Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (DT, VT, TU, MS)
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (MS)

Received March 10 2012; Revised June 11 2012; Accepted June 15 2012
Advance Acess publication July 21 2012
DOI 10.1093/jxb/ers197

Figure 1. The effect of myrigalone A (MyA) on Lepidium sativum seed germination in continuous light and in darkness.
(a) The effect of different concentrations of MyA on the time required for germinating seed populations to reach 50% testa rupture (TR50%) or endosperm rupture (ER50%) displayed as dose responses.
(b) Endosperm cap puncture force (red circles) and endosperm rupture kinetics during germination without (CON) or with 5x10-4 M MyA added (MyA).
(c) Endosperm rupture kinetics of germinating seeds, incubated in continuous white light without (CON) and with 5x10-4 M MyA preincubated for 35h in darkness or continuous light.
Mean values ± SE of ≥ 3 replicates of 50 seeds each are shown for testa and endosperm rupture; mean values ± SE of 10-50 seeds for the puncture-force measurements.

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Supplementary data file (2 MB)

Abstract
Supplementary:

Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Fig. 7
Fig. S1 Fig. S2 Fig. S3 Fig. S4 Table S1

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Journal of Experimental Botany 63: 5337-5350 (2012)

Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A [W][OA]

**Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A** ^[W]^[OA]