The Seed Biology Place

Journal of Experimental Botany 63: 5337-5350 (2012)

**Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A** ^[W]^[OA]

Antje Voegele, Kai Graeber, Krystyna Oracz, Danuse Tarkowská, Dominique Jacquemoud, Veronika Turecková, Terezie Urbanová, Miroslav Strnad, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, German (AV, KG, KO, DJ, GLM)
School of Biological Sciences, Plant Molecular Science, Royal Holloway, University of London, Egham, Surrey TW20 0EX, Uk (AV, KG, GLM); Web: 'The Seed Biology Place' - www.seedbiology.eu
Department of Plant Physiology, Warsaw University of Life Sciences-SGGW, 15 Nowoursynowska 159, 02-776, Warsaw, Poland (KO)
Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, v.v.i., Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (DT, VT, TU, MS)
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (MS)

Received March 10 2012; Revised June 11 2012; Accepted June 15 2012
Advance Acess publication July 21 2012
DOI 10.1093/jxb/ers197

Figure 6. The effect of MyA on testa characteristics.
(a) Kinetics of testa rupture of Lepidium sativum during seed germination without (CON) and with 5x10-4 M MyA added, and incubated in continuous light with 0, 10, 20 and 30mM PEG.
(b-d) Testa permeability test using the tetrazolium assay. Entire seeds were incubated in tetrazolium staining solution for 3h (b) and 15h (d) without (CON) and with 5x10-4 M MyA (MyA) together with 0 and 30 mM PEG, embryos were extracted and staining was scored.
(c) Embryos extracted from 3h-CON and MyA-treated seeds were stained as positive controls.
(e) The relationship between embryo sizes and testa rupture (onset and TR50%) for CON and MyA at different PEG concentrations.
Mean values ± SE of 50 embryos.

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Supplementary data file (2 MB)

Abstract
Supplementary:

Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Fig. 7
Fig. S1 Fig. S2 Fig. S3 Fig. S4 Table S1

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Journal of Experimental Botany 63: 5337-5350 (2012)

Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A [W][OA]

**Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A** ^[W]^[OA]