The Seed Biology Place

Journal of Experimental Botany 63: 5337-5350 (2012)

**Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A** ^[W]^[OA]

Antje Voegele, Kai Graeber, Krystyna Oracz, Danuse Tarkowská, Dominique Jacquemoud, Veronika Turecková, Terezie Urbanová, Miroslav Strnad, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, German (AV, KG, KO, DJ, GLM)
School of Biological Sciences, Plant Molecular Science, Royal Holloway, University of London, Egham, Surrey TW20 0EX, Uk (AV, KG, GLM); Web: 'The Seed Biology Place' - www.seedbiology.eu
Department of Plant Physiology, Warsaw University of Life Sciences-SGGW, 15 Nowoursynowska 159, 02-776, Warsaw, Poland (KO)
Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, v.v.i., Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (DT, VT, TU, MS)
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (MS)

Received March 10 2012; Revised June 11 2012; Accepted June 15 2012
Advance Acess publication July 21 2012
DOI 10.1093/jxb/ers197

Figure 5. The contribution of different embryo parts to embryo mask area increase during Lepidium sativum seed germination.
(a) Workflow for quantification of COT and RAD mask areas. The raw images were digitally dissected at the apical meristem with a 90° angle towards the stem into a ‘cotyledon’ (COT) part and into a ‘radicle/hypocotyl-axis’ (RAD) part. COT and RAD masks were created and their areas quantified as shown in Fig 2a.
(b) Increase in COT and RAD mask areas of embryos extracted from seeds incubated without (CON) or with 5x10-4 M MyA (MyA). The relative increase of the COT and RAD sizes from 3h to the time for ER50% for CON (22h) and MyA (35h) was calculated from 50 embryo raw images taken from seeds incubated in continuous light for each indicated time point.
Mean values are presented; SE<1% for CON and MyA-COT, 7% for MyA-RAD.

Article in PDF format (1.5 MB)
Supplementary data file (2 MB)

Abstract
Supplementary:

Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Fig. 7
Fig. S1 Fig. S2 Fig. S3 Fig. S4 Table S1

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Journal of Experimental Botany 63: 5337-5350 (2012)

Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A [W][OA]

**Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A** ^[W]^[OA]