Proteomics 10: 406-416 (2010)

Proteomics reveal tissue-specific features of the cress (Lepidium sativum L.) endosperm cap proteome and its hormone-induced changes during seed germination [W]
Kerstin Müller, Claudette Job, Maya Belghazi, Dominique Job, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de (K.M., G.L.-M.)
Centre National de la Recherche Scientifique-Université Claude Bernard Lyon-Institut National des Sciences Appliquées-Bayer CropScience Joint Laboratory (UMR 5240), Bayer CropScience, Lyon, France (C.J., D.J.)
Centre d'Analyse Protéomique de Marseille, Institut Fédératif de Recherche Jean Roche, Marseille, France (M.B.)

Received July 29, 2009; revised November 3, 2009; accepted November 5, 2009
DOI 10.1002/pmic.200900548
Supplemental Table S4. Identified proteins from the cap of germinating cress seeds belonging to functional category 11–disease and defense.

 

fold change

 

 

spot #

8 to
18

18 to
18 ruptured

8 to
18 ABA

18 ABA to
96 ABA

protein name

functional category

154

0.59

0.83

0.53

0.20

12-oxophytodienoate reductase

11.02 defense regulated

222

4.58

0.51

0.87

0.51

thiocyanate forming protein

11.02 defense regulated

224

1.77

1.09

0.43

1.40

thiocyanate forming protein

11.02 defense regulated

242

1.01

0.50

0.89

3.11

GF14 psi chain

11.05 stress response

279

0.72

1.84

0.88

1.02

ATPER1 (1-cysteine peroxiredoxin 1)

11.05 stress response

195

5.82

0.68

3.04

2.16

aldo/keto reductase family protein

11.06 detoxification

287

3.57

1.31

2.25

1.83

glutathione S-transferase

11.06 detoxification

293

0.63

0.83

1.34

1.75

glutathione transferase

11.06 detoxification

295

1.00

0.86

1.10

0.62

glutathione transferase

11.06 detoxification

296

0.87

3.24

0.92

0.90

glutathione transferase

11.06 detoxification


Supplemental Table S4. Identified proteins from the cap of germinating cress seeds belonging to functional category 11–disease and defense.
Only spots in which just one protein was identified are listed.
“Fold change” is calculated from the averaged means of normalized spot volumes of three 2D-gels loaded with biological replicates.
Bold numbers indicate statistically significant differences at p<0.05 in a t-test.
For detailed information on spot characteristics, refer to Supplemental Table S1 and Supplemental Fig. S3.
Article in PDF format (365 KB)
Supplemental data file (5 MB)
Abstract

 Figure 1         Figure 2         Figure 3         Figure 4
Suppl. Figures:     S1     S2     S3     S4
Suppl. Tables:      S1     S2     S3     S4		  
© Wiley InterScience
WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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