Proteomics 10: 406-416 (2010)

Proteomics reveal tissue-specific features of the cress (Lepidium sativum L.) endosperm cap proteome and its hormone-induced changes during seed germination ^[W]

Supplemental Table S1. List of all identified proteins from endosperm caps of germinating cress seeds. Proteins were analyzed by two-dimensional electrophoresis and identified by LC/MS-MS.

Supplemental Table S1 (provided as Excel file) - download (111 KB)

N° = spot label on the reference maps presented in Supplemental Figures S1-S4;
8 h CON (av volume) = average normalized spot volume, calculated from three analyzed gels with extracts from biological replicates of 1000-2000 cress endosperm caps from seeds imbibed for 8 h;
18 h CON (av volume) = average normalized spot volume, calculated from three analyzed gels with extracts from biological replicates of 1000-2000 INTACT cress endosperm caps from seeds imbibed for 18 h;
18 h CON ruptured (av volume) = average normalized spot volume, calculated from three analyzed gels with extracts from biological replicates of 1000-2000 RUPTURED cress endosperm caps from seeds imbibed for 18 h;
18 h ABA (av volume); 96 h ABA (av volume) = average normalized spot volume, calculated from three analyzed gels with extracts from biological replicates of 1000-2000 cress endosperm caps from seeds imbibed for 18 h and 96 h, respectively, in the presence of 10 µM ABA;
SD = standard deviation;
fold change = calculated from the averaged means of normalized spot volumes of three 2D-gels loaded with biological replicates. Red numbers indicate statistically significant differences at p< 0.05 in a t-test;
Number of id per spot, number of proteins identified in the corresponding spot; quantitation of spot volumes is only presented for spots containing a single protein;
Cluster, cluster 1, proteins whose accumulation level remained constant; cluster 2, proteins whose accumulation level varied after endosperm rupture; clusters 3 and 4, proteins whose accumulation level varied prior to endosperm rupture in water (cluster 3) or ABA (cluster 4);
Exp MM (Da) = experimental molecular mass;
Exp pI = experimental isoelectric point;
Theo MM (Da) = theoretical molecular mass;
Theo pI = theoretical isoelectric point;
Organism = organism in which the protein has been identified;
No. NCBI accession = accession number in NCBI database;
Real Mascot matched queries = number of unique peptides identified with Mascot software;
Peptides = list of peptides identified;
Mascot score = identification score obtained with Mascot software;
Mascot coverage % = percentage of sequence coverage obtained with identified peptides with Mascot software for the orthologous protein;
Function category and Function description = functional categories defined according to the ontological classification of Bevan et al. [Bevan et al. (1998) Nature 391:485-488].