The Seed Biology Place

Proteomics 10: 406-416 (2010)

**Proteomics reveal tissue-specific features of the cress (Lepidium sativum L.) endosperm cap proteome and its hormone-induced changes during seed germination** ^[W]

Kerstin Müller, Claudette Job, Maya Belghazi, Dominique Job, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de (K.M., G.L.-M.)
Centre National de la Recherche Scientifique-Université Claude Bernard Lyon-Institut National des Sciences Appliquées-Bayer CropScience Joint Laboratory (UMR 5240), Bayer CropScience, Lyon, France (C.J., D.J.)
Centre d'Analyse Protéomique de Marseille, Institut Fédératif de Recherche Jean Roche, Marseille, France (M.B.)

Received July 29, 2009; revised November 3, 2009; accepted November 5, 2009
DOI 10.1002/pmic.200900548

Supplemental Table S2. Identified proteins from the cap of germinating cress seeds belonging to functional category 02–energy.

	fold change
spot #	8 to 18	18 to 18 ruptured	8 to 18 ABA	18 ABA to 96 ABA	protein name	functional category
168	2.97	0.63	0.53	3.92	fructose-bisphosphate aldolase, putative	02.01 glycolysis
169	2.47	0.42	0.58	2.17	fructose-bisphosphate aldolase, cytoplasmic isozyme	02.01 glycolysis
174	1.12	0.52	2.22	3.91	fructose-bisphosphate aldolase	02.01 glycolysis
176	2.65	0.78	3.31	1.30	fructose-bisphosphate aldolase, putative	02.01 glycolysis
253	0.66	1.06	0.60	0.74	triosephosphate isomerase, cytosolic	02.01 glycolysis
180	0.68	1.07	1.22	1.84	putative glyceraldehyde-3-phosphate dehydrogenase	02.01 glycolysis
185	0.75	1.11	0.78	0.55	glyceraldehyde-3-phosphate dehydrogenase	02.01 glycolysis
186	2.01	1.10	1.36	1.16	glyceraldehyde-3-phosphate dehydrogenase	02.01 glycolysis
190	2.29	0.48	1.41	1.81	glyceraldehyde-3-phosphate dehydrogenase	02.01 glycolysis
173	1.13	0.41	1.40	1.24	PGK (phosphoglycerate kinase)	02.01 glycolysis
192	3.17	0.18	2.92	1.07	PGK (phosphoglycerate kinase)	02.01 glycolysis
7	1.26	0.50	1.09	1.57	cytoplasmic aconitate hydratase	02.02 gluconeogenesis
191	0.87	0.66	0.68	3.52	malate dehydrogenase, cytosolic, putative	02.02 gluconeogenesis
210	0.72	1.20	1.20	0.90	malate dehydrogenase	02.10 TCA
212	0.82	1.00	0.92	1.03	malate dehydrogenase (NAD), mitochondrial	02.10 TCA

Supplemental Table S2. Identified proteins from the cap of germinating cress seeds belonging to functional category 02–energy.
Only spots in which just one protein was identified are listed.
“Fold change” is calculated from the averaged means of normalized spot volumes of three 2D-gels loaded with biological replicates.
Bold numbers indicate statistically significant differences at p<0.05 in a t-test.
For detailed information on spot characteristics, refer to Supplemental Table S1 and Supplemental Fig. S1.

Article in PDF format (365 KB)
Supplemental data file (5 MB)

Abstract

Figure 1         Figure 2         Figure 3         Figure 4
Suppl. Figures:     S1     S2     S3     S4
Suppl. Tables:      S1     S2     S3     S4

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Proteomics 10: 406-416 (2010)

Proteomics reveal tissue-specific features of the cress (Lepidium sativum L.) endosperm cap proteome and its hormone-induced changes during seed germination [W]

**Proteomics reveal tissue-specific features of the cress (Lepidium sativum L.) endosperm cap proteome and its hormone-induced changes during seed germination** ^[W]