The Seed Biology Place

Proteomics 10: 406-416 (2010)

**Proteomics reveal tissue-specific features of the cress (Lepidium sativum L.) endosperm cap proteome and its hormone-induced changes during seed germination** ^[W]

Kerstin Müller, Claudette Job, Maya Belghazi, Dominique Job, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de (K.M., G.L.-M.)
Centre National de la Recherche Scientifique-Université Claude Bernard Lyon-Institut National des Sciences Appliquées-Bayer CropScience Joint Laboratory (UMR 5240), Bayer CropScience, Lyon, France (C.J., D.J.)
Centre d'Analyse Protéomique de Marseille, Institut Fédératif de Recherche Jean Roche, Marseille, France (M.B.)

Received July 29, 2009; revised November 3, 2009; accepted November 5, 2009
DOI 10.1002/pmic.200900548

Supplemental Table S3. Identified proteins from the cap of germinating cress seeds belonging to functional category 06–protein folding and stability.

	fold change
spot #	8 to 18	18 to 18 ruptured	8 to 18 ABA	18 ABA to 96 ABA	protein name	functional category
17	2.01	0.13	1.51	0.73	SHD (SHEPHERD); HSP 90-like	06.01 folding and stability
23	2.44	1.05	1.88	1.29	protein disulfide isomerase precursor-like	06.01 folding and stability
24	0.89	0.37	1.30	0.57	heat shock cognate protein 80	06.01 folding and stability
276	1.71	1.75	1.07	0.94	chaperonin 10	06. folding and stability
290	0.47	2.53	0.92	1.05	chaperonin 10	06.01 folding and stability
236	2.82	0.77	1.31	1.52	aspartyl protease family protein	06.13 proteolysis
266	0.99	0.76	1.46	3.63	aspartyl protease family protein	06.13 proteolysis
223	1.88	1.30	1.13	1.10	26S proteasome regulatory subunit, putative	06.13 proteolysis
278	3.06	4.60	1.22	1.50	Cruciferin CRU1 precursor (11S globulin)	06. storage protein
301	0.85	2.16	0.67	0.22	CRU3 (CRUCIFERIN 3)	06.20 storage protein
302	0.82	2.32	0.23	2.44	CRU3 (CRUCIFERIN 3)	06.20 storage protein
317	1.37	0.49	1.07	0.62	CRA1 (CRUCIFERIN A)	06.20 storage protein
297	0.33	0.57	0.12	0.91	seed storage protein beta-chain 6 (fragment)	06.20 storage protein

Supplemental Table S3. Identified proteins from the cap of germinating cress seeds belonging to functional category 06–protein folding and stability.
Only spots in which just one protein was identified are listed.
“Fold change” is calculated from the averaged means of normalized spot volumes of three 2D-gels loaded with biological replicates.
Bold numbers indicate statistically significant differences at p<0.05 in a t-test.
For detailed information on spot characteristics, refer to Supplemental Table S1 and Supplemental Fig. S2.

Article in PDF format (365 KB)
Supplemental data file (5 MB)

Abstract

Figure 1         Figure 2         Figure 3         Figure 4
Suppl. Figures:     S1     S2     S3     S4
Suppl. Tables:      S1     S2     S3     S4

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Proteomics 10: 406-416 (2010)

Proteomics reveal tissue-specific features of the cress (Lepidium sativum L.) endosperm cap proteome and its hormone-induced changes during seed germination [W]

**Proteomics reveal tissue-specific features of the cress (Lepidium sativum L.) endosperm cap proteome and its hormone-induced changes during seed germination** ^[W]