Journal of Experimental Botany 63: 6325-6334 (2012)
Role of a respiratory burst oxidase of Lepidium sativum (cress) seedlings in root development and auxin signalling [W][OA]
Simon Fraser University, Department of Biological Sciences, 8888 University Drive, Burnaby BC V5A 1S6, Canada (KM, ARK)
University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, German (AL, GLM)
School of Biological Sciences, Plant Molecular Science, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK (GLM);
Web: 'The Seed Biology Place' - www.seedbiology.eu
* equal contributions
Received 20 July 2012; Revised 11 September 2012; Accepted 21 September 2012
Advance Acess publication 23 October 2012
DOI 10.1093/jxb/ers284
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Fig. 2. Expression analysis of Lesarbohs and their Arabidopsis respiratory burst oxidase orthologues. Transcript abundance analysis for five members of the gene family determined by qRT-PCR in different plant tissues of L. sativum and A. thaliana. Note the similarities in intensity and expression profile between the two species. Averages of three biological replicates ± SE are presented. For the imbibed seed samples, seeds were harvested after 24 h on water in continuous light. Other samples included 5-d-old seedlings (5 d post-imbibition), and mature adult plants harvested 4 weeks post-germination. No AtrbohA transcript was found in any of the stages tested. Transcript abundance was corrected to the abundance of transcripts associated with the standard genes - the constitutive genes Actin 7 (ACT7; At5g09810) and Elongation Factor 1α (EF1α; At5g60390) for Arabidopsis and the corresponding genes (ACT7, HQ436350.1 and EF1α, HS981853.1) for L. sativum. n.d.=not detectable. |
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