Darwin Review - Journal of Experimental Botany 62: 3289-3309 (2011)
First off the mark: early seed germination
Department of Biological Sciences, Simon Fraser University, 8888, University Drive, Burnaby BC, V5A 1S6, Canada (K.M.)
*Joint first authors: K.W., K.M.
Received December , 2010; accepted February 23, 2010; published online April 12, 2010
DOI 10.1111/j.1469-8137.2010.03249.x
Supplementary Table 1. Transcriptome analysis for energy metabolism genes during Arabidopsis thaliana germination sensu strictu and its regulation by hormones and cold stratification.
The seed-specific eFP-browser and the eNorthern tool at www.bar.utoronto.ca were used to analyse transcript expression patterns based on global transcriptome analyses during Arabidopsis seed germination (Winter et al., 2007; Bassel et al., 2008). The time course of transcriptional changes of key metabolic enzyme genes during the early phase of germination (dry seed (0h) to 24h) of non-dormant, non-stratified Arabidopsis Col seeds are listed as relative values (Nakabayashi et al., 2005; Preston et al., 2009); in addition the absolute values in dry seeds and at 6h are listed (in red). The imbibition-mediated transcript expression patterns the ratios 6h/dry were calculated and classified as follows: up-regulation if ratio ≥2, equal if ratio 0.5 to 2, down-regulation if ratio ≤0.5, up24 if up-regulation is only evident at 24h. The transcript regulation patterns by moist cold-stratification were determined from the ratios 4ºC / 22ºC of seeds imbibed in darkness for 96h (Yamauchi et al., 2004). The regulation by gibberellins (GA) was analysed by calculating the ratios obtained with 6h-imbibed GA-deficient ga1-3 seeds ±GA (RIKEN transcriptome sets at www.bar.utoronto.ca). The regulation by abscisic acid (ABA) was analysed by calculating the ratios obtained with 24h-imbibed Ler seeds ±ABA (RIKEN transcriptome sets at www.bar.utoronto.ca). The column "proteome analysis" contains the corresponding protein expression patterns for germination sensu strictu compiled from the following publications: (1) Arabidopsis whole seed, germination sensu strictu after cold-stratification (Fu et al. 2005). (2) Arabidopsis whole seed, germination sensu strictu, www.seedproteome.com. (3) Lepidium sativum endosperm cap, germination sensu strictu, no cold-stratification (Müller et al. 2010). (4) Arabidopsis whole seed ratio, germination sensu strictu, α-amanitin/control (Rajjou et al. 2004). (5) Arabidopsis whole seed, germination sensu strictu (Gallardo et al. 2001).
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