Plant Physiology 109: 751-759 (1995)

Class I ß-1,3-glucanases in the endosperm of tobacco during germination

Gerhard Leubner-Metzger, Corinne Fründt, Regina Vögeli-Lange (1), Frederick Meins, Jr.

Table I  Localization of ß-1,3-glucanase and chitinase expression in stage II germinating seeds just prior to endosperm rupture Seed component a ß-1,3-Glucanase GLB promoter activity Chitinase   Control ABA Control ABA Control ABA   pkat/seed fkat/seed b pkat/seed Entire seed 2.37 ± 0.26 c 0.56 ± 0.11 1.63 ± 0.43 0.12 ± 0.05 0.30 ± 0.05 0.29 ± 0.07 Embryo 0.03 ± 0.02 0.04 ± 0.02 0.03 ± 0.02 ca. 0 d 0.14 ± 0.04 0.12 ± 0.01 Entire endosperm 2.57 ± 0.27 0.56 ± 0.18 1.94 ± 0.46 0.09 ± 0.06 0.19 ± 0.05 0.23 ± 0.01 Micropylar endosperm 2.67 ± 0.06 0.69 ± 0.31 1.30 ca. 0 0.08 ± 0.01 0.11 ± 0.08 Non-micropylar endosperm 0.11 ± 0.03 ca. 0 ca. 0 ca. 0 0.07 ± 0.05 0.05 ± 0.04 a Batches of 100 seeds were dissected after 3 d incubation in continuous light with 10-5 M ABA (ABA) and without ABA (Control). The endosperm fractions contain a thin layer of adhering seed coat. b Expressed as GUS activity measured using seeds of a homozygous GLB-GUS transformant c Mean values ± SEM for at least two independent experiments d below the limit of detection, 0.004

Article in PDF format (2.5 MB) Abstract           Fig. 1          Fig. 2          Fig. 3          Fig. 4          Fig. 5          Table 1

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