Plant Physiology 109: 751-759 (1995)

Class I ß-1,3-glucanases in the endosperm of tobacco during germination

Gerhard Leubner-Metzger, Corinne Fründt, Regina Vögeli-Lange (1), Frederick Meins, Jr.

Figure 1. The time course of ß-1,3-glucanase induction during tobacco seed germination. (a) The incidence of seed-coat (testa) rupture () and endosperm rupture () expressed as % of 100-200 seeds scored with time after the start of imbibition. The activities of ß-1,3-glucanase () and chitinase (), are expressed in pkat/seed. The values presented are the means ± SEM obtained with at least two batches of seed in two independent experiments. (b) The time course for accumulation of class I ß-1,3-glucanase mRNA () and GUS activity () resulting from expression of the class I GLB promoter. The content of mRNA is expressed in arbitrary PhosphoImager units per seed. A similar pattern of induction was found when mRNA was normalized for the signal obtained with 18S rRNA. GUS activity, expressed as fkat/seed, was measured in seeds from plants homozygous for the GLB-GUS transgene. The time course for covering-layer rupture and ß-1,3- glucanase activity was the same for GLB-GUS and wild-type seed lots.

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