Plant Molecular Biology 38: 785-795 (1998)
Ethylene responsive element binding protein (EREBP) expression
and the transcriptional regulation of class I ß-1,3-glucanase
during tobacco seed germination
| Incubation conditions |
Seed component 1 |
ß-1,3-Glucanase (pkat / seed) |
GUS (fkat / seed) |
Endosperm rupture (%) |
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| Control | Ethylene | Control | Ethylene | Control | Ethylene | ||
| 54 h light | 0.2 ± 0.2 | 8.2 ± 0.8 | |||||
| Embryo | 0.12 ±0.002 | 0.16 ± 0.00 | 0.02 ± 0.01 | 0.02 ± 0.00 | |||
| Endosperm | 1.22 ± 0.02 | 2.07 ± 0.11 | 0.61 ± 0.03 | 1.59 ± 0.16 | |||
| 65 h light 3 | |||||||
| No addition | Entire seed | 1.65 ± 0.09 | 3.00 ± 0.31 | 1.00 ± 0.14 | 3.27 ± 0.36 | 79.3 ± 3.5 | 87.5 ± 1.6 |
| 1 mM AVG | Entire seed | 0.62 ± 0.07 | 1.32 ± 0.02 | 0.25 ± 0.09 | 0.67 ± 0.00 | 12.6 ± 4.0 | 59.1 ± 0.6 |
| 50 ppm NBD | Entire seed | 1.04 ± 0.10 | 2.43 ± 0.00 | 0.59 ± 0.05 | 1.91 ± 0.06 | 17.2 ± 2.6 | 62.8 ± 0.4 |
| 80h darkness | 3.5 ± 0.9 | 4.7 ± 0.7 | |||||
| Entire dormant seed | 0.10 ± 0.01 | 0.17 ± 0.05 | 0.02 ± 0.01 | 0.03 ± 0.00 | |||
| Entire non-dormant seed | 1.45 ± 0.31 | 3.40 ± 0.92 | 0.30 ± 0.02 | 0.93 ± 0.19 | |||
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1 Batches of 100 dissected or entire seeds were assayed. Samples of embryos and endosperms were obtained from seeds with intact endosperms. Note that dormant seeds have intact testas and intact endosperms.
2 Mean values ± SE for at least two independent experiments.
3 Testa rupture ≥ 96 % in all 65 h light samples.
Hyperlink to drawing of the tobacco class I ß-1,3-glucanase B (GLB) gene promoter
| Article in PDF format (2.1 MB) | Abstract Fig. 1 Fig. 2 Fig. 3 Table 1 GLB-promoter |
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