Plant Molecular Biology 38: 785-795 (1998)
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Ethylene responsive element binding protein (EREBP) expression
and the transcriptional regulation of class I ß-1,3-glucanase
during tobacco seed germination
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Gerhard Leubner-Metzger, Luciana Petruzzelli (1), Rosa Waldvogel,
Regina Vögeli-Lange (2), Frederick Meins, Jr.
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Friedrich-Miescher Institute, Box 2543, CH-4002 Basel, Switzerland
(1) Istituto Biosintesi Vegetali, C.N.R., Via Bassini 15, I-20133 Milano,
Italy
(2) Present address: Botanical Institute, University of Basel, Hebelstrasse
1, CH-4056 Basel, Switzerland
Received: 14 November 1997 / Accepted: 20 May 1998
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Abstract. Class I ß-1,3-glucanase (ßGLU I) is
transcriptionally induced in the micropylar endosperm just before its rupture
prior to the germination (i.e., radicle emergence) of Nicotiana tabacum
L. cv "Havana 425" seeds. Ethylene is involved in endosperm rupture and
high-level ßGLU I expression; but, it does not affect the spatial
and temporal pattern of ßGLU I expression. A promoter deletion analysis
of the tobacco ßGLU I B gene suggests that (1) the distal -1452 to
-1193 region, which contains the positively-acting ethylene-responsive element
(ERE), is required for high-level, ethylene-sensitive expression, (2) the
regions -1452 to -1193 and -402 to 0 contribute to down-regulation by abscisic
acid (ABA), and (3) the region -402 to -211 is necessary and sufficient
for low-level micropylar-endosperm specific expression. Transcripts of the
ERE binding proteins (EREBPs) showed a novel pattern of expression during
seed germination: light or gibberellin was required for EREBP-3 and EREBP-4
expression; EREBP-4 expression was constitutive and unaffected by ABA or
ethylene; EREBP-3 showed transient induction just before endosperm rupture,
which was earlier in ethylene-treated seeds and inhibited by ABA. No expression
of EREBP-1 and EREBP-2 was detected. In contrast to ßGLU I, EREBP-3
and EREBP-4 were not expressed specifically in the micropylar endosperm.
The results suggest that transcriptional regulation of ßGLU I could
depend on: activation of ethylene signalling pathways acting via EREBP-3
with the ERE as the target, and ethylene-independent signalling pathways
with targets in the proximal promoter region that are likely to determine
spatial and temporal patterns of expression.
Key words: endosperm, Nicotiana, ß-1,3-glucanase, ethylene,
ethylene-responsive element, DNA binding protein, signal transduction, seed
dormancy
Hyperlink to drawing of the tobacco class I ß-1,3-glucanase B (GLB) gene promoter
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