The Seed Biology Place

Journal of Experimental Botany 61: 491-502 (2010)

**Peroxidases identified in a subtractive cDNA library approach show tissue-specific transcript abundance and enzyme activity during seed germination of Lepidium sativum ^[W]^[OA]**

Ada Linkies, Uta Schuster-Sherpa, Stefanie Tintelnot, Gerhard Leubner-Metzger, Kerstin Müller

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de

Received August 27, 2009; revised October 6, 2009; accepted October 12, 2009
Advance Acess publication 30 October 2009
DOI 10.1093/jxb/erp318

Table 2. Percentages of testa and endosperm rupture at selected times after imbibition for fresh and after-ripened seeds of Arabidopsis WT and SALKlines that showed a germination phenotype in the screen.
Endosperm rupture of imbibed seeds was recorded over time at 24°C in continuous light. Means of three replicates of 50 seeds +/- SE are given. AR = after-ripened, nd = not determined. All differences in endosperm rupture to the WT are statistically significant at P<0.05 in the after-ripened and at P<0.01 in the fresh state.

Mutated gene	Line	Fresh (48 h)		AR (41 h)
		Testa rupture (%)	Endosperm rupture (%)	Testa rupture (%)	Endosperm rupture (%)
	WT Columbia	52±4	45±8	83±7	76±6
Aspartyl protease	SALK_021485.37.45.x	24±2	18±3	58±1	49±1
PP2C	SALK_016641.56.00.x	22±5	13±3	26±6	22±5
UBI11	SALK_069877.55.25.x	19±4	15±4	47±6	39±4
UBI14	SALK_107827.16.05.x	25±2	17±3	49±6	44±5
AtPrxIIE	SALK_064512.50.75.x	19±2	13±2	nd	nd
AtPrx16	SALK_028328.55.00.x	79±4	71±3	nd	nd
AtAPX01	SALK_000249.38.45.x	100±0	91±3	nd	nd

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Abstract

Figure 1         Figure 2         Figure 3
Table 1          Table 2          Suppl. data file

Suppl. Figure S1
Suppl. Table S1

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Journal of Experimental Botany 61: 491-502 (2010)

Peroxidases identified in a subtractive cDNA library approach show tissue-specific transcript abundance and enzyme activity during seed germination of Lepidium sativum [W][OA]

**Peroxidases identified in a subtractive cDNA library approach show tissue-specific transcript abundance and enzyme activity during seed germination of Lepidium sativum ^[W]^[OA]**