Journal of Experimental Botany 61: 491-502 (2010)

Peroxidases identified in a subtractive cDNA library approach show tissue-specific transcript abundance and enzyme activity during seed germination of Lepidium sativum [W][OA]

Ada Linkies, Uta Schuster-Sherpa, Stefanie Tintelnot, Gerhard Leubner-Metzger, Kerstin Müller

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de

Received August 27, 2009; revised October 6, 2009; accepted October 12, 2009
Advance Acess publication 30 October 2009
DOI 10.1093/jxb/erp318

Table 2. Percentages of testa and endosperm rupture at selected times after imbibition for fresh and after-ripened seeds of Arabidopsis WT and SALKlines that showed a germination phenotype in the screen.
Endosperm rupture of imbibed seeds was recorded over time at 24°C in continuous light. Means of three replicates of 50 seeds +/- SE are given. AR = after-ripened, nd = not determined. All differences in endosperm rupture to the WT are statistically significant at P<0.05 in the after-ripened and at P<0.01 in the fresh state.

Mutated gene

Line

Fresh (48 h)

AR (41 h)

 

 

Testa
rupture (%)

Endosperm
rupture (%)

Testa
rupture (%)
Endosperm
rupture (%)

 

WT Columbia

52±4

45±8

83±7

76±6

Aspartyl protease

SALK_021485.37.45.x

24±2

18±3

58±1

49±1
PP2C
SALK_016641.56.00.x
22±5
13±3
26±6
22±5
UBI11
SALK_069877.55.25.x
19±4
15±4
47±6
39±4
UBI14
SALK_107827.16.05.x
25±2
17±3
49±6
44±5
AtPrxIIE
SALK_064512.50.75.x
19±2
13±2
nd
nd
AtPrx16
SALK_028328.55.00.x
79±4
71±3
nd
nd
AtAPX01
SALK_000249.38.45.x
100±0
91±3
nd
nd

Article in PDF format (780 KB)
(includes Suppl. data)
Abstract

Figure 1         Figure 2         Figure 3
Table 1          Table 2          Suppl. data file
         
Suppl. Figure S1
Suppl. Table S1
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Society of Experimental Botany
http://jxb.oxfordjournals.org/
 

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