Journal of Experimental Botany 61: 491-502 (2010)

Peroxidases identified in a subtractive cDNA library approach show tissue-specific transcript abundance and enzyme activity during seed germination of Lepidium sativum [W][OA]

Ada Linkies, Uta Schuster-Sherpa, Stefanie Tintelnot, Gerhard Leubner-Metzger, Kerstin Müller

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de

Received August 27, 2009; revised October 6, 2009; accepted October 12, 2009
Advance Acess publication 30 October 2009
DOI 10.1093/jxb/erp318

Linkies Fig. 3
Figure 3. Transcript levels of the three peroxidases identified in the subtractive cDNA library in different Lepidium sativum (cress) seed tissues and in dry micropylar seed ends (R+C) at different points during germination (in h) in the absence (CON = control) and presence of 10 µM ABA. Transcript abundance was determined by qPCR and normalized against actin abundance. Note the different scales on the Y-axes. Means of three biological replicates ±SE are shown. Cap = micropylar endosperm cap, Nme = non-micropylar endosperm, Cot = cotyledons.


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