Journal of Experimental Botany 58: 3047-3060 (2007)

1-Aminocyclopropane-1-carboxylic acid and abscisic acid during the germination of sugar beet (Beta vulgaris L.) - a comparative study of fruits and seeds

Katrin Hermann, Juliane Meinhard, Peter Dobrev, Ada Linkies, Bedrich Pesek, Barbara Heß, Ivana Machácková, Uwe Fischer, Gerhard Leubner-Metzger

Institute of Biology II, Botany / Plant Physiology, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, D-79104 Freiburg i. Br., Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de (K.H., A.L., B.H., G-L.-M.) KWS SAAT AG, Grimsehlstr. 31, D-37555 Einbeck, Germany, Web: http://www.kws.de (J.M., U.F.) Institute of Experimental Botany CAS, Rozvojová 263, CZ-16502 Prague 6, Czech Republic, Web: http://www.ueb.cas.cz/index_eng.htm (P.D., B.P., I.M.) Received June 15, 2007; accepted June 25, 2007

Figure 6. The effects of abscisic acid (ABA) and 1-aminocyclopropane-1-carboxylic acid (ACC, ethylene precursor) on the transcript expression of ACC oxidase (ACO, ethylene-forming enzyme) and ABA 8'-hydroxylase (CYP707A, ABA-degrading enzyme), ACC and ABA leaching into the medium, ethylene evolution and endogenous ACC and ABA of either seeds dissected from incubated fruits (left) or incubated 'isolated seeds' (right) of Beta vulgaris (sugar beet). RT-PCR was performed from total RNA extracted from seeds at the incubation time T50% (see Fig. 5). Specific primers for conserved regions of the known Beta vulgaris ACO (BI095869, NCBI database, http://www.ncbi.nlm.nih.gov) and CYP707A (BQ582685, NCBI database) mRNA sequences were used that resulted into the expected amplification of 340-bp and 201-bp bands, respectively; 18S rRNA primers amplifing 0.45 kb PCR bands served as internal controls. The endogenous ABA and ACC contents of seeds at T50% were calculated from Fig. 5 (dry seed mass = 3.6 ± 0.4 mg; a similar volume in µl was determined). Ethylene evolution at T50% was determined in the time window between 48h and 62h; nd = not detected, i.e. below the detection limit. ABA and ACC leaching into the medium was determined prior to radicle emergence (prior to T1%) in the time window between 0h and 30h. For comparison, the ABA and ACC concentrations of hormone additions to the medium are presented in the last rows. These experiments were performed with the experimental setup described in Figs. 4 and 5.

Article in PDF format (1.3 MB)    Abstract    Fig. 1               Fig. 2 Fig. 5               Fig. 6 Fig. 3               Fig. 4                Tab. 1 Fig. 7               Suppl. Fig. S1 © Oxford University Press, Oxford Journals Society of Experimental Botany http://jxb.oxfordjournals.org/

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