Journal of Experimental Botany 58: 3047-3060 (2007)
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1-Aminocyclopropane-1-carboxylic acid and abscisic acid during the germination of sugar beet (Beta vulgaris L.) - a comparative study of fruits and seeds
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Katrin Hermann, Juliane Meinhard, Peter Dobrev, Ada Linkies, Bedrich Pesek, Barbara Heß, Ivana Machácková, Uwe Fischer, Gerhard Leubner-Metzger
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Institute of Biology II, Botany / Plant Physiology, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, D-79104 Freiburg i. Br., Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de (K.H., A.L., B.H., G-L.-M.) KWS SAAT AG, Grimsehlstr. 31, D-37555 Einbeck, Germany, Web: http://www.kws.de (J.M., U.F.) Institute of Experimental Botany CAS, Rozvojová 263, CZ-16502 Prague 6, Czech Republic, Web: http://www.ueb.cas.cz/index_eng.htm (P.D., B.P., I.M.) Received June 15, 2007; accepted June 25, 2007 |
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Figure 2. Time courses of visible events during the germination of Beta vulgaris (sugar beet). (A) Population responses of fruits and (botanically true) seeds. Fruits: Triplicates of 100 fruits incubated at 15 ºC in the dark were scored over time and the percentage of operculum opening (ovary cap lifting, Fig. 1B,E) and radicle protrusion (completion of seed germination, Fig. 1C,D,F,G,H) of the fruit populations were calculated. Seeds: Triplicates of 25 seeds were scored for radicle protrusion. Mean values ± SE of three (fruits) and six (seeds) independent experiments are presented. SE values < 1.5 % are not drawn. (B) Population responses of DCM-permeated fruits and seeds. Dry fruits were permeated with dichloromethane (DCM; Control-perm) or DCM plus cycloheximide (CHX-perm). Scoring, conditions, and statistics of one (fruits) and two (seeds) independent experiments as in (A). |
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