Planta 199: 282-288 (1999)

Effects of gibberellins, darkness and osmotica on endosperm rupture and class I ß-1,3-glucanase induction in tobacco seed germination

Gerhard Leubner-Metzger, Corinne Fründt, Frederick Meins, Jr.
Table 1. Effect of GA4 on the accumulation and localization of ß-1,3-glucanase and GUS activity in dark-incubated seeds prior to endosperm rupture.

 

ß-1,3-Glucanase b

GUS c

Seed component a

Control

+GA4

Control

+GA4

Entire seed

0.03 ± 0.01 d

2.63 ± 0.28

0.07

0.48

Embryo

n.d. e

0.02

n.d.

<0.001

Entire endosperm

n.d.

3.00

n.d.

0.48

Micropylar endosperm

n.d.

2.98

n.d.

0.48

Non-micropylar endosperm

n.d.

0.02

n.d.

<0.001

a GA4 treated seeds were dissected after 3 d incubation just before the onset of endosperm rupture; as controls, entire photodormant seeds were measured after 3 d incubation
b data obtained with 100 wild-type seeds expressed as pkat per seed
c data obtained with 100 homozygous GLB-GUS seeds expressed as fkat per seed
d values obtained for two or more experiments are expressed as the mean ±SE
e not determined
Article in PDF format (2.5 MB) Abstract          Fig. 1          Fig. 2          Fig. 3          Tab. 1  
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