Planta 199: 282-288 (1999)

Effects of gibberellins, darkness and osmotica on endosperm rupture and class I ß-1,3-glucanase induction in tobacco seed germination

Gerhard Leubner-Metzger, Corinne Fründt, Frederick Meins, Jr.
glucanase in tobacco seeds Fig. 2A,B. The effect of 10-5 M GA4 and 10-5 M ABA on the accumulation of class I ß-1,3-glucanase antigen and mRNA in dark-incubated seeds.

(A) Immunoblot analysis of antigens reacting with antibody directed against tobacco class I ß-1,3-glucanase. Equal amounts of protein (80 µg) were applied to each lane. GLN I, 10 ng of purified tobacco ß-1,3-glucanase. The apparent size in kDa of immunoreactive bands is indicated.

(B) RNA-blot analysis of total RNA (30 µg per lane) prepared from photodormant (D) and non-photodormant (ND) subpopulations of seeds incubated in the dark. The hybridization probe used is for tobacco class I ß-1,3-glucanase mRNA. The size of the transcript in kb is indicated

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