Planta 213: 758-763 (2001)

Brassinosteroids and gibberellins promote tobacco seed germination by distinct pathways

Gerhard Leubner-Metzger

Table 1. Effect of BR and GA on seed germination, seedling growth and ßGLU activity induction of non-photodormant tobacco seeds

 

 

Germinating seeds

 

Seedlings

 

 

Light

 

Darkness

 

Light

Treatment

 

Endosperm
rupture

ßGLU
activity

 

Endosperm
rupture

ßGLU
activity

 

Hypocotyl
growth

 

 

 

 

 

Control

 

29.4 ± 1.6 a

2.2 ± 0.2 b

 

34.7 ± 2.9

0.7 ± 0.1

 

100 ± 3 c

0.01 µM BL

 

93.0 ± 0.4

2.2 ± 0.1

 

72.1 ± 3.8

0.6 ± 0.1

 

127 ± 2

10 µM GA4

 

27.6 ± 2.4

2.1 ± 0.2

 

80.2 ± 1.4

2.3 ± 0.3

 

128 ± 2

0.01 µM BL + 10 µM GA4

 

94.1 ± 0.9

n.d. d

 

84.8 ± 2.2

n.d.

 

146 ± 3

10 µM ABA

 

0.0 ± 0.0

0.4 ± 0.1

 

0.0 ± 0.0

0.2 ± 0.1

 

n.d.

0.01 µM BL + 10 µM ABA

 

0.0 ± 0.0

0.2 ± 0.1

 

0.0 ± 0.0

0.2 ± 0.1

 

n.d.

10 µM GA4 + 10  µM ABA

 

0.0 ± 0.0

n.d.

 

0.0 ± 0.0

0.6 ± 0.1

 

n.d.


a Mean ± SE of % endosperm rupture of at least two populations of ca. 100 non-photodormant seeds imbibed for 55 h in continuous light or for 65 h in darkness
b ßGLU activity expressed as pkat per seed
c Mean ± SE of % control hypocotyl growth of at least 20 de-etiolated seedlings germinated for 150 h in darkness and then grown for 100 h in continuous light
d Not determined

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