Planta 215: 959-968 (2002)

Seed after-ripening and over-expression of class I ß-1,3-glucanase confer maternal effects on tobacco testa rupture and dormancy release

Gerhard Leubner-Metzger
Table 1. Effect of tobacco seed after-ripening and sense ßGlu I transformation on testa rupture, endosperm rupture and ßGlu enzyme activity of reciprocal crosses
  50 % Testa rupture (continuous light)   50 % Endosperm rupture (continuous light)
Time (h) b ßGlu (pkat/seed) d  
Time (h) b
 ßGlu (pkat/seed) d
Crosses (Lines) a

   'Fresh'

 After-
ripened		 Differ-
ence f		 'Fresh'

 After-
ripened		   'Fresh'

 After-
ripened		 Differ-
ence f		 'Fresh'

 After-
ripened
                                         
TKSG7-32 x   37.3 (37.5) c 34.5 3.3 1.5   65.6 62.0 7.4 5.2
TKSG7-38 x   42.0 34.3 2.7 1.7   68.9 62.7 7.6 4.8
TKSG7-43 x   --- 34.7 --- 2   --- 61.4 --- 5.3
TKSG7 x (Mean)   39.6 34.5 5.1 3.0 1.7   67.2   62.0   5.2   7.5   5.1
TKSG7-32 x WT   36.5 (36.0) 36.0 1.3 0.8   69.9 63.3 4.2 2.4
TKSG7-38 x WT   43.4 (40.0) 34.3 2.2 1.6   68.3 62.4 5.6 2.8
TKSG7-43 x WT   --- 35.2 --- 1.2   --- 63.8 --- 5.1
TKSG7 x WT (Mean)   39.9 35.2 4.8 1.7 1.2   69.1 63.2 5.9 4.9 3.5
---
WT x TKSG7-32   51.4 (50.0) 37.0 0.3 0.2   76.6 64.8 2.9 2.2
WT x TKSG7-38   50.2 (49.5) 34.8 0.8 0.2   77.9 63.0 4.1 2.5
WT x TKSG7-43   --- 35.4 --- 0.8   --- 63.5 --- 3.2
WT x TKSG7 (Mean)   50.8 35.7 15 0.6 0.4   77.3 63.8 13.5 3.5 2.6
TCIB1-2 x   50.2 (47.0) 34.3 0.2 0.2   76.9 62 2.6 2.5
TCIB1-2 x WT   51.8 34.2 --- 0.1   77.6 62.9 2.6 2.3
WT x TCIB1-2   49.5 (49.0) 36.3 0.2 0.2   77.7 62.8 2.4 2.3
TCIB1-2 / WT (Mean)   50.5 34.9 15.6 0.2 0.2   77.4   62.6   14.8   2.5   2.4
               

Hyperlink to figure presenting the core results of the table
a Reciprocal crosses of independent, monogenic vector-control (TCIB1) or sense-ßGlu I (TKSG7) tobacco lines with wild type (WT); female parent in cross is on the left and the male parent is not shown in self crosses; "(Mean)" designates mean values of presented data obtained for the independent lines;
b Time to reach 50 % testa or endosperm rupture, respectively, determined from the time-course of seeds imbibed in continuous light in control medium; 100-150 'fresh' (directly after harvest) or after-ripened (about one year of dry storage) seeds from four independent capsules were scored at each time-point; time-course data for TKSG7-32 and relevant statistics are presented in Fig. 1
c Time to reach 50 % testa rupture in the presence of 10 µM ABA is shown in parenthesis
d ßGlu enzyme activities at the time of 50 % testa or endosperm rupture, respectively, determined in protein extracts from seed samples described under b
e Not determined
f  Time differences of 'fresh' and afterripened seeds to reach 50 % testa or endosperm rupture, respectively

© Leubner-Metzger, Planta 215: 959-968 (2002)
Article in PDF format (316 KB)
Abstract Fig. 1 Fig. 2 Fig. 3 Table 1          Maternal   crosses
© Springer
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