Plant Physiology 161: 1903-1917 (2013)

Spatiotemporal seed development analysis provides insight into primary dormancy induction and evolution of the Lepidium DELAY OF GERMINATION1 Genes [W][OA]
Kai Graeber, Antje Voegele, Annette Büttner-Mainik, Katja Sperber, Klaus Mummenhoff, Gerhard Leubner-Metzger

School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (KG, AV, GLM);
Web: 'The Seed Biology Place' - www.seedbiology.eu
University of Freiburg, Faculty of Biology, Institute for Biology II, Botany/Plant Physiology, D-79104 Freiburg, Germany (KG, AV, ABM, GLM)
Universität Osnabrück, Fachbereich Biologie, Botanik, D-49069 Osnabrück, Germany (KS, KM)

Received December 24, 2012; Accepted Feburary 19, 2013; Published Feburary 20, 2013.
DOI:10.1104/pp.112.213298

Supplemental Table S2. Primer sequences used for cloning of Lepidium DOG1 gene sequences and primer pair combinations (indicated by a common letter) as used in Supplemental Table S1.

Primer name

Primer Sequence (5’-3’)

Primer pair combination
1-exFP1-DOG1

ACCAAGAGTGGATGAATTTGC

C
1-exFP2-DOG1

CGCGTACCTGATCTCAAACA

B
2-exRP2-DOG1

CGGCGACGATCTCTCATAGT

A, B, D
viPCR-FP2

ACCGGATTCGACATTATACCA

D
iPCR-RP2

CAAACCATCGATGTCACGAA

C
LoxyDOG1a FP2

TTATGCGCCGTCTTGGAACA

E
LoxyDOG1a RP2

GCAAGAAATTGGCCGCTTCG

E
LoxyDOG1b FP1

TTACGCCGGAAGAAGAGCTG

F
LoxyDOG1b RP1

TGCTTGTCGAGAGCTTGGTC

F
viPCR-FP1

CCAAAACACAAAACACAGCA

A
LesaDOG1a-FP3-wgDNA

TATGTATTTTAGAAAAATGGGATCTTC

  
LesaDOG1-FP1-GW

GAAGGAGATAGAACCATGGGATCTTCAAAGAAGAACATCG

  
LesaDOG1-RP1-GW

CTATTGCTTCTTCTCCTCCTCTCCTTTGG

  
GSP-3

AGCCACACAAAGCATAAACGA

  

Article in PDF format (2 MB)
Supplementary data file (4.9 MB)
Abstract

 Fig. 1         Fig. 2         Fig. 3         Fig. 4         Fig. 5         Fig. 6         Fig. 7
Fig. S1       Fig. S2       Fig. S3      Fig. S4       Table S1    Table S2
  
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