Planta 235: 137-151 (2012)
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Dose- and tissue-specific interaction of monoterpenes with the gibberellin-mediated release of potato tuber bud dormancy, sprout growth and induction of α-amylases and β-amylases
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Sonja Rentzsch • Dagmara Podzimska • Antje Voegele • Madeleine Imbeck • Kerstin Müller§ • Ada Linkies* • Gerhard Leubner-Metzger*
*shared corresponding authors
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University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de
§Department of Biological Sciences, Simon Fraser University, 9 8888, University Drive, Burnaby BC, V5A 1S6, Canada
Received 11 June 2011; Accepted 5 August 2011;
Published online 20 August 2011
DOI: 10.107/s00425-011-1501-1
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Fig. 1 The effect of gibberellin on potato tuber bud dormancy release, sprout growth and on the expression of starch hydrolases.
(a) Tuber discs containing dormant buds (‘eyes’) were excised from dormant tubers of Solanum tuberosum cv. Agria and shaken for 2h in a solution without (CON, control) and with 50 µM gibberellin A3 added (GA). Tuber discs were subsequently incubated on wet filter paper for one week and dissected into sprout (S) and base (B) tissue for subsequent hydrolase analyses. Tuber disc base (B) tissue contains periderm ('skin') and proximal 'sub-eye' cortex tissue.
(b) The effect of GA treatment on tuber disc bud dormancy release and sprout growth. Bud dormancy release was scored by calculating the percentage of tuber discs with visible sprouts (≥ 2 mm in length). Sprout growth was measured daily as sprout length.
(c) The α-amylase, β-amylase, and α-glucosidase enzyme activities of sprouts from GA-treated tuber discs were analysed over time using specific assays.
(d) The enzyme activities from unsprouted bud tuber disc tissue (CON) was measured for comparison. Mean values ± SE (n≥3 tuber discs for activities, n≥10 tuber discs for sprouting). Insert: The relative transcript abundances of α-amylases, β-amylases, and α-glucosidases in GA-treated tuber bud discs were obtained from the transcriptome analysis of Hartmann et al. (2011): Transcript abundance values are presented as fold regulation (day3/day0) for the α-AMY2-group α-amylase amyA1 (MICRO.10377.C2), the β-amylases MICRO.13368.C1 (2) and POACG68TP (3), and the α-glucosidases MICRO.856.C1 (4), SSBT006B09x.scf (5) and cSTA12F14TH (6)
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