Methods in Molecular Biology 773: 319-327 (2011)

In vivo ¹H-NMR microimaging during seed imbibition, germination, and early growth

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de (G. L.-M.)
Department of Biological Sciences, Simon Fraser University, 9 8888, University Drive, Burnaby BC, V5A 1S6, Canada (K. M., A. K.)
Steacie Institute for Molecular Sciences, National Research Council Canada, Ottawa, ON, Canada (V. T.)

Chapter 18 in: Allison R. Kermode (ed.), Seed Dormancy: Methods and Protocols
DOI 10.1007/978-1-61779-231-1_18, © Springer Science+Business Media, LLC 2011

Fig. 2. In vivo 1H-NMR chemical shift selective imaging (CSSI) of western white pine seed during germination.

The spatial distribution of water (upper row) and oil (lower row) simultaneously mapped in the same seed over a course of early germination (left to right) is visualized with false colors as shown.
Coronal plane 2D MRI images were obtained with a 78 mm in-plane resolution and a 500-mm slice thickness.