The Seed Biology Place

The Plant Cell 21: 3803-3822 (2009)

**Ethylene interacts with abscisic acid to regulate endosperm rupture during germination: a comparative approach using Lepidium sativum and Arabidopsis thaliana ^[W][OA]**

Ada Linkies, Kerstin Müller, Karl Morris, Veronika Turecková, Meike Wenk, Cassandra S. C. Cadman, Françoise Corbineau, Miroslav Strnad, James R. Lynn, William E. Finch-Savage, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de (A.L., Ke.M., M.W., G.L.-M.)
Warwick Horticulture Research International (HRI), Warwick University, Wellesbourne, Warwick CV35 9EF, United Kingdom (Ka.M., C.S.C.C., J.R.L., W.E.F.-S.)
Palacky University and Institute of Experimental Botany Academy of Sciences of the Czech Republic, Laboratory of Growth Regulators, CZ-78371 Olomouc, Czech Republic (V.T., M.S.)
Université Pierre et Marie Curie-Paris 6, Germination et Dormance des Semences, UR5, Site d'Ivry, F-75005 Paris, France (F.C.)

Received July 23, 2009; Returned for revision October 12, 2009; Accepted November 17, 2009; Published December 18, 2009
www.plantcell.org/cgi/doi/10.1105/tpc.109.070201

Supplemental Data Files

The following materials are available in the online version of this article.

Supplemental Figures, Tables and Methods (1.8 MB PDF).

Supplemental Dataset 1 (12.8 MB Excel file). Normalized mean expression values transcripts used in the analysis of the CON-arrays (22025 gene list).

Supplemental Dataset 2 (11.9 MB Excel file). Normalized mean expression values transcripts used in the analysis of the ABA-arrays (19794 gene list).

Supplemental Dataset 3 (26.5 MB Excel file). Statistical (t-tests, F-tests) and TAGGIT analysis of the CON-arrays transcripts (22025 gene list).

Supplemental Dataset 4 (24.4 MB Excel file). Statistical (t-tests, F-tests) and TAGGIT analysis of the ABA-arrays transcripts (19794 gene list).

Supplemental Dataset 5 (0.8 MB Excel file). Significantly regulated transcripts in the CON-arrays (1350 gene list) based on adjusted p-values (≤ 0.1) from F-Tests.

Supplemental Dataset 6 (1.8 MB Excel file). Significantly regulated transcripts in the ABA-arrays (3530 gene list) based on adjusted p-values (≤ 0.1) from F-Tests.

Supplemental Dataset 7 (1.4 MB Excel file). Venn diagram comparison of transcripts regulated in Arabidopsis seedlings by ethylene and/or ABA and in the Lepidium seed CON-arrays.

Supplemental Dataset 8 (0.9 MB Excel file). Venn diagram comparison of transcripts regulated in Arabidopsis seedlings by ethylene and/or ABA and in the Lepidium seed ABA-arrays.

Supplemental Dataset 9 (2.1 MB fasta format). Nucleotide sequences used to generate the phylogenetic tree presented in Figure 7D.

Synopsis: Tissue weakening of the endosperm is required to allow radicle protrusion during seed germination. Cross-species work including tissue-specific transcriptome analysis and biomechanical measurement of endosperm weakening provided a new mechanistic model that explains how ethylene promotes seed germination and counteracts the inhibition of endosperm cap weakening by abscisic acid.

Article in PDF format (1.8 MB)
Supplemental data file (1.8 MB)
Abstract of Plant Cell 2009

Fig. 1
Fig. 6
Fig. S1

Fig. 2
Fig. 7
Fig. S2

Fig. 3
Fig. 8
Fig. S3

Fig. 4
Fig. 9
Fig. S4

Fig. 5
Fig. 10
Fig. S5

Hyperlink to
Supplemental
Datasets 1 to 9

Webdesign Gerhard Leubner 2000
Best viewed with browser version 4 and 800x600 pixel
This page was last updated on 30 January, 2010

The Plant Cell 21: 3803-3822 (2009)

Ethylene interacts with abscisic acid to regulate endosperm rupture during germination: a comparative approach using Lepidium sativum and Arabidopsis thaliana [W][OA]

**Ethylene interacts with abscisic acid to regulate endosperm rupture during germination: a comparative approach using Lepidium sativum and Arabidopsis thaliana ^[W][OA]**