Physiologia Plantarum 86: 495-501 (1992)

Hydroxycinnamoylputrescines are not causally involved in the tuberization process in potato plants

Gerhard Leubner-Metzger and Nikolaus Amrhein

Institute of Plant Sciences, Plant Biochemistry and Physiology, Swiss Federal Institute of Technology, Sonneggstr. 5, CH-8092 Zürich, Switzerland Received: 28 July 1992

Table 1. Comparison of the endogenous levels of caffeoylputrescine (CP), feruloylputrescine (FP), diferuloylputrescine (DFP), free putrescine (Put) and free spermidine (Spd) in field grown potato plants (data of Leubner-Metzger and Amrhein 1992) and in leaf cuttings of S. tuberosum ssp. tuberosum cv. Astrid, in in vitro stolon cultures of S. tuberosum ssp. tuberosum cv. Indira, and in in vitro minituber cultures of S. tuberosum ssp. tuberosum cv. Carola and of S. tuberosum ssp. andigena 482W (this work). Data on the levels of basic HCAs (CP, FP, pCP and traces of caffeoylspermidine) in leaf cuttings of the F1-potato hybrid SDA6 are from Paynot et al. (1983). BAP, benzylaminopurine; ND, not determined. Experimental system Levels of HCAs and free polyamines   µmol (g dry weight)-1 CP FP DFP Put Spd     Field grown plants cv. Astrid    unthickened stolon tips 0.007 0.001 0.001 2.6 3.4    thickened stolon tips 0.013 0.004 0.001 1.2 2.4    young tubers < 0.5 cm 0.017 0.007 0.001 0.8 1.5    young tubers 0.5-2 cm 0.008 0.005 0.000 0.9 1.1     Leaf cuttings cv. Astrid (in nutrient solution)    tuberizing axillary buds (without BAP) 0.00 0.02 0.001 ND ND    tuberizing axillary buds (with 22 µM BAP) 0.13 0.57 0.090 ND ND     In vitro stolon culture cv. Indira    untuberized culture (without BAP) 0.017 0.195 ND ND ND    tuberized culture (with 22 µM BAP) 0.072 0.477 ND ND ND       In vitro minituber culture cv. Carola    Culture day 32 (without BAP) 0.100 0.264 ND ND ND    Culture day 32 (with 22 µM BAP) 1.926 1.432 ND ND ND       In vitro minituber culture ssp. andigena 482W    Culture day 0 0.007 0.067 0.006 1.7 0.7    Culture day 9 (without BAP) 0.018 0.154 0.013 0.4 0.1    Culture day 9 (with 22 µM BAP) 0.190 0.288 0.029 0.8 0.3    Culture day 27 (without BAP) 0.018 0.257 0.006 0.1 0.0    Culture day 27 (with 22 µM BAP) 0.111 0.351 0.010 0.1 0.0     Total levels of basic HCAs (CP, FP, pCP, traces of caffeoylspermidine) µmol (g fresh weight)-1 Leaf cuttings of the F1-potato hybrid SDA6    tuberized stolons (short day) 0.020    untuberized stolons (long day) 0.002

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