Figure 3. Tissue specificity and regulation by ABA of ß-1,3-glucanase (ßGlu) and chitinase (Chn) activities during the seed germination of Capsicum annuum, measured in
(B) micropylar cap and
(C) the rest of the seed.
Populations of 20 seeds were imbibed in continuous light, either in the absence (Control) or presence of 100 μM ABA (ABA), and seeds were dissected at the times indicated. Specific ßGlu and Chn activities are expressed in pkat/μg protein. Mean values ± SE are presented from four to ten replicate samples at each time point. Radicle protrusion of imbibed pepper seeds: 0% until 72 h, 11.6% at 96 h, 100% at 120 h (Control) and 0% for ABA-treated seeds at 120 h. At 96 h non-germinated (N) and germinated (G) seeds were measured separately.