Plant, Cell and Environment 26: 661-671 (2003)

Calcium requirement for ethylene-dependent responses involving 1-aminocyclopropane-1-carboxylic acid oxidase in radicle tissues of germinated pea seeds

Additional Figure (unpublished data). Effects of calcium antagonists on the ethylene-mediated induction in the radicle of germinated pea seeds of 34.5 kDa ß-1,3-glucanase antigen accumulation.

The 48 h-imbibed pea seeds were pre-incubated for 2 h in the presence or absence of the calcium antagonists followed by a further incubation for 8 h exposure to 30 mL/L ethylene in the presence or absence of the calcium antagonists as described in Fig. 3; RR (100 mM), W-7 (100 mM), Li (50 mM), Ca (10 mM), Neo (50 mM). For furter details about the ß-1,3-glucanase antigen see Petruzzelli et al. (1999).